Genopole Evry


Accueil > Research > Physiology and Signalling > J. Colcombet Team


Stress signalling

- de Zelicourt A., Colcombet J., Hirt H. (2016). The Role of MAPK Modules and ABA during Abiotic Stress Signaling. Trends Plant Sci. 21(8):677-85. doi : 10.1016/j.tplants.2016.04.004.

- Colcombet J., Sözen C. , Hirt H. (2016). Convergence of Multiple MAP3Ks on MKK3 Identifies a Set of Novel Stress MAPK Modules. Front. Plant Sci. 7:1941. doi : 10.3389/fpls.2016.01941.



- Boudsocq M., Danquah A., de Zélicourt A., Hirt H., Colcombet J. (2015). Plant MAPK cascades : just rapid signaling modules ? Plant Signal Behav. 10(9):e1062197. doi : 10.1080/15592324.2015.1062197.

- Guzel Deger, A., Scherzer, S., Nuhkat, M., Kedzierska, J., Kollist, H., Brosché, M., Unyayar, S., Boudsocq, M., Hedrich, R., Roelfsema, M. R. (2015). Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure. New Phytol. 208(1):162-73. doi : 10.1111/nph.13435.

- Bigeard J, Colcombet J, Hirt H. (2015). Signaling Mechanisms in Pattern-Triggered Immunity (PTI).​ Mol. Plant. 8(4):521–39. doi : 10.1016/j.molp.2014.12.022.

- Danquah A, de Zelicourt A, Boudsocq M, Neubauer J ,Frey NFD, Leonhardt N, Pateyron S, Gwinner F, Tamby JP, Ortiz-Masia D, Marcote MJ,Hirt H, Colcombet J. (2015). Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana. Plant J. 82 (2) : 232-44.

- Li G, Boudsocq M, Hem S, Vialaret J, Rossignol M, Maurel C, Santoni V. (2015). The calcium-dependent protein kinase CPK7 acts on root hydraulic conductivity. Plant Cell Environ. 38(7):1312-20. doi : 10.1111/pce.12478.



- Bigeard, J., Rayapuram, N., Pflieger, D., and Hirt, H. (2014) Phosphorylation-dependent regulation of plant chromatin and chromatin-associated proteins. Proteomics 14, 2127-2140.

- Bigeard, J., Rayapuram, N., Bonhomme, L., Hirt, H., and Pflieger, D. (2014) Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics 14, 2141-2155.

- Bigeard, J., Pflieger, D., Colcombet, J., Gérard, G., Mireau, H., and Hirt, H. (2014) Protein Complexes Characterization in Arabidopsis thaliana by Tandem Affinity Purification Coupled to Mass Spectrometry Analysis. Methods Mol Biol 1171:237-250.

- Frei dit Frey, N., Garcia, A.V., Bigeard, J., Zaag, R., Bueso, E., Garmier, M., Pateyron, S., de Tauzia-Moreau, M.L., Brunaud, V., Balzergue, S., Colcombet, J., Aubourg, S., Martin-Magniette, M.L., and Hirt, H. (2014) Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences. Genome Biol 15, R87.

- Garcia, A.V., Charrier, A., Schikora, A., Bigeard, J., Pateyron, S., de Tauzia-Moreau, M.L., Evrard, A., Mithofer, A., Martin-Magniette, M.L., Virlogeux-Payant, I., and Hirt, H. (2014) Salmonella enterica flagellin is recognized via FLS2 and activates PAMP-triggered immunity in Arabidopsis thaliana. Mol Plant 7, 657-674.

- Hudik, E., Berriri, S., Hirt, H., and Colcombet, J. (2014) Identification of constitutively active AtMPK6 mutants using a functional screen in Saccharomyces cerevisiae. Methods Mol Biol 1171, 67-77.

- Neumann, C., Fraiture, M., Hernandez-Reyes, C., Akum, F.N., Virlogeux-Payant, I., Chen, Y., Pateyron, S., Colcombet, J., Kogel, K.H., Hirt, H., Brunner, F., and Schikora, A. (2014) The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells. Front Microbiol 5, 548.

- Ovecka, M., Takac, T., Komis, G., Vadovic, P., Bekesova, S., Doskocilova, A., Samajova, V., Luptovciak, I., Samajova, O., Schweighofer, A., Meskiene, I., Jonak, C., Krenek, P., Lichtscheidl, I., Skultety, L., Hirt, H., and Samaj, J. (2014) Salt-induced subcellular kinase relocation and seedling susceptibility caused by overexpression of Medicago SIMKK in Arabidopsis. J Exp Bot 65, 2335-2350.

- Rayapuram, N., Bonhomme, L., Bigeard, J., Haddadou, K., Przybylski, C., Hirt, H., and Pflieger, D. (2014) Identification of novel PAMP-triggered phosphorylation and dephosphorylation events in Arabidopsis thaliana by quantitative phosphoproteomic analysis. J Proteome Res 13, 2137-2151.

- Zourelidou, M., Absmanner, B., Weller, B., Barbosa, I.C., Willige, B.C., Fastner, A., Streit, V., Port, S.A., Colcombet, J., de la Fuente van Bentem, S., Hirt, H., Kuster, B., Schulze, W.X., Hammes, U.Z., and Schwechheimer, C. (2014) Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. Elife 3.



- Colcombet, J., Berriri, S., and Hirt, H. (2013) Constitutively active MPK4 helps to clarify its role in plant immunity. Plant Signal Behav 8.
Danquah, A., de Zelicourt, A., Colcombet, J., and Hirt, H. (2013) The role of ABA and MAPK signaling pathways in plant abiotic stress responses. Biotechnol Adv 32, 40-52.

- de Zelicourt, A., Al-Yousif, M., and Hirt, H. (2013) Rhizosphere microbes as essential partners for plant stress tolerance. Mol Plant 6, 242-245.

- Evrard, A., Kumar, M., Lecourieux, D., Lucks, J., von Koskull-Doring, P., and Hirt, H. (2013) Regulation of the heat stress response in Arabidopsis by MPK6-targeted phosphorylation of the heat stress factor HsfA2. PeerJ 1, e59.

- Khan, M., Rozhon, W., Bigeard, J., Pflieger, D., Husar, S., Pitzschke, A., Teige, M., Jonak, C., Hirt, H., and Poppenberger, B. (2013) Brassinosteroid-regulated GSK3/Shaggy-like kinases phosphorylate mitogen-activated protein (MAP) kinase kinases, which control stomata development in Arabidopsis thaliana. J Biol Chem 288, 7519-7527.

- Latrasse, D., Jegu, T., Meng, P.H., Mazubert, C., Hudik, E., Delarue, M., Charon, C., Crespi, M., Hirt, H., Raynaud, C., Bergounioux, C., and Benhamed, M. (2013) Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator. Nucleic Acids Res 41, 2907-2917.

- Montillet, J.-L., and Hirt, H. (2013) New checkpoints in stomatal defense. Trends in Plant Science.

- Montillet, J.L., Leonhardt, N., Mondy, S., Tranchimand, S., Rumeau, D., Boudsocq, M., Garcia, A.V., Douki, T., Bigeard, J., Lauriere, C., Chevalier, A., Castresana, C., and Hirt, H. (2013) An abscisic Acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis. PLoS Biol 11, e1001513.

- Smeets, K., Opdenakker, K., Remans, T., Forzani, C., Hirt, H., Vangronsveld, J., and Cuypers, A. (2013) The role of the kinase OXI1 in cadmium- and copper-induced molecular responses in Arabidopsis thaliana. Plant Cell Environ 36, 1228-1238.



- Berriri, S., Garcia, A.V., Frei Dit Frey, N., Rozhon, W., Pateyron, S., Leonhardt, N., Montillet, J.L., Leung, J., Hirt, H., and Colcombet, J. (2012) Constitutively Active Mitogen-Activated Protein Kinase Versions Reveal Functions of Arabidopsis MPK4 in Pathogen Defense Signaling. Plant Cell.

- Garcia, A.V., Al-Yousif, M., and Hirt, H. (2012) Role of AGC kinases in plant growth and stress responses. Cell Mol Life Sci 69, 3259-3267.

- Hirt, H., Garcia, A.V., and Oelmuller, R. (2012) AGC kinases in plant development and defense. Plant Signal Behav 6, 1030-1033.
Schikora, A., Garcia, A.V., and Hirt, H. (2012) Plants as alternative hosts for Salmonella. Trends Plant Sci 17, 245-249.

- Schikora, M., Neupane, B., Madhogaria, S., Koch, W., Cremers, D., Hirt, H., Kogel, K.H., and Schikora, A. (2012) An image classification approach to analyze the suppression of plant immunity by the human pathogen Salmonella Typhimurium. BMC Bioinformatics 13, 171.

- Vandenbogaert, M., Hourdel, V., Jardin-Mathe, O., Bigeard, J., Bonhomme, L., Legros, V., Hirt, H., Schwikowski, B., and Pflieger, D. (2012) Automated phosphopeptide identification using multiple MS/MS fragmentation modes. J Proteome Res 11, 5695-5703.



- Camehl, I., Drzewiecki, C., Vadassery, J., Shahollari, B., Sherameti, I., Forzani, C., Munnik, T., Hirt, H., and Oelmuller, R. (2011) The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis. PLoS Pathog 7, e1002051.

- Forzani, C., Carreri, A., de la Fuente van Bentem, S., Lecourieux, D., Lecourieux, F., and Hirt, H. (2011) The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases. Febs J 278, 1126-1136.

- Pflieger, D., Bigeard, J., and Hirt, H. (2011) Isolation and characterization of plant protein complexes by mass spectrometry. Proteomics 11, 1824-1833.

- Pflieger, D., Gonnet, F., de la Fuente van Bentem, S., Hirt, H., and de la Fuente, A. (2011) Linking the proteins—elucidation of proteome-scale networks using mass spectrometry. Mass Spectrom Rev 30, 268-297.

- Samajova, O., Plihal, O., Al-Yousif, M., Hirt, H., and Samaj, J. (2011) Improvement of stress tolerance in plants by genetic manipulation of mitogen-activated protein kinases. Biotechnol Adv 31, 118-128.

- Schikora, A., Virlogeux-Payant, I., Bueso, E., Garcia, A.V., Nilau, T., Charrier, A., Pelletier, S., Menanteau, P., Baccarini, M., Velge, P., and Hirt, H. (2011) Conservation of Salmonella infection mechanisms in plants and animals. PLoS One 6, e24112.



- Kosetsu, K., Matsunaga, S., Nakagami, H., Colcombet, J., Sasabe, M., Soyano, T., Takahashi, Y., Hirt, H., and Machida, Y. (2010) The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana. Plant Cell 22, 3778-3790.

- Pitzschke, A., and Hirt, H. (2010) New insights into an old story : Agrobacterium-induced tumour formation in plants by plant transformation. Embo J 29, 1021-1032.

- Pitzschke, A., and Hirt, H. (2010) Mechanism of MAPK-targeted gene expression unraveled in plants. Cell Cycle 9, 18-19.

- Pitzschke, A., and Hirt, H. (2010) Bioinformatic and systems biology tools to generate testable models of signaling pathways and their targets. Plant Physiol 152, 460-469.



- Bartels, S., Anderson, J.C., Gonzalez Besteiro, M.A., Carreri, A., Hirt, H., Buchala, A., Metraux, J.P., Peck, S.C., and Ulm, R. (2009) MAP kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and SNC1-mediated responses in Arabidopsis. Plant Cell 21, 2884-2897.

- de la Fuente van Bentem, S., and Hirt, H. (2009) Protein tyrosine phosphorylation in plants : More abundant than expected ? Trends Plant Sci 14, 71-76.

- Pecinka, A., Rosa, M., Schikora, A., Berlinger, M., Hirt, H., Luschnig, C., and Mittelsten Scheid, O. (2009) Transgenerational stress memory is not a general response in Arabidopsis. PLoS One 4, e5202.

- Pitzschke, A., and Hirt, H. (2009) Disentangling the complexity of mitogen-activated protein kinases and reactive oxygen species signaling. Plant Physiol 149, 606-615.

- Pitzschke, A., Schikora, A., and Hirt, H. (2009) MAPK cascade signalling networks in plant defence. Curr Opin Plant Biol 12, 421-426.

- Pitzschke, A., Djamei, A., Bitton, F., and Hirt, H. (2009) A major role of the MEKK1-MKK1/2-MPK4 pathway in ROS signalling. Mol Plant 2, 120-137.

- Pitzschke, A., Djamei, A., Teige, M., and Hirt, H. (2009) VIP1 response elements mediate mitogen-activated protein kinase 3-induced stress gene expression. Proc Natl Acad Sci U S A 106, 18414-18419.



- Anderson, J.F., Parrish, T.D., Akhtar, M., Zurek, L., and Hirt, H. (2008) Antibiotic resistance of enterococci in American bison (Bison bison) from a nature preserve compared to that of Enterococci in pastured cattle. Appl Environ Microbiol 74, 1726-1730.

- Chen, P.Y., Lee, K.T., Chi, W.C., Hirt, H., Chang, C.C., and Huang, H.J. (2008) Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants. Planta 228, 499-509.

- Colcombet, J., and Hirt, H. (2008) Arabidopsis MAPKs : a complex signalling network involved in multiple biological processes. Biochem J 413, 217-226.

- de la Fuente van Bentem, S., Mentzen, W.I., de la Fuente, A., and Hirt, H. (2008) Towards functional phosphoproteomics by mapping differential phosphorylation events in signaling networks. Proteomics 8, 4453-4465.

- de la Fuente van Bentem, S., Anrather, D., Dohnal, I., Roitinger, E., Csaszar, E., Joore, J., Buijnink, J., Carreri, A., Forzani, C., Lorkovic, Z.J., Barta, A., Lecourieux, D., Verhounig, A., Jonak, C., and Hirt, H. (2008) Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis. J Proteome Res 7, 2458-2470.

- Pieroni, E., de la Fuente van Bentem, S., Mancosu, G., Capobianco, E., Hirt, H., and de la Fuente, A. (2008) Protein networking : insights into global functional organization of proteomes. Proteomics 8, 799-816.

- Schikora, A., Carreri, A., Charpentier, E., and Hirt, H. (2008) The dark side of the salad : Salmonella typhimurium overcomes the innate immune response of Arabidopsis thaliana and shows an endopathogenic lifestyle. PLoS One 3, e2279.



- Brader, G., Djamei, A., Teige, M., Palva, E.T., and Hirt, H. (2007) The MAP kinase kinase MKK2 affects disease resistance in Arabidopsis. Mol Plant Microbe Interact 20, 589-596.

- Daxberger, A., Nemak, A., Mithofer, A., Fliegmann, J., Ligterink, W., Hirt, H., and Ebel, J. (2007) Activation of members of a MAPK module in beta-glucan elicitor-mediated non-host resistance of soybean. Planta 225, 1559-1571.

- de la Fuente van Bentem, S., and Hirt, H. (2007) Using phosphoproteomics to reveal signalling dynamics in plants. Trends Plant Sci 12, 404-411.

- Djamei, A., Pitzschke, A., Nakagami, H., Rajh, I., and Hirt, H. (2007) Trojan horse strategy in Agrobacterium transformation : abusing MAPK defense signaling. Science 318, 453-456.

- Doczi, R., Brader, G., Pettko-Szandtner, A., Rajh, I., Djamei, A., Pitzschke, A., Teige, M., and Hirt, H. (2007) The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling. Plant Cell 19, 3266-3279.

- Kemmerling, B., Schwedt, A., Rodriguez, P., Mazzotta, S., Frank, M., Qamar, S.A., Mengiste, T., Betsuyaku, S., Parker, J.E., Mussig, C., Thomma, B.P., Albrecht, C., de Vries, S.C., Hirt, H., and Nurnberger, T. (2007) The BRI1-associated kinase 1, BAK1, has a brassinolide-independent role in plant cell-death control. Curr Biol 17, 1116-1122.

- Kempa, S., Rozhon, W., Samaj, J., Erban, A., Baluska, F., Becker, T., Haselmayer, J., Schleiff, E., Kopka, J., Hirt, H., and Jonak, C. (2007) A plastid-localized glycogen synthase kinase 3 modulates stress tolerance and carbohydrate metabolism. Plant J 49, 1076-1090.

- Schweighofer, A., Kazanaviciute, V., Scheikl, E., Teige, M., Doczi, R., Hirt, H., Schwanninger, M., Kant, M., Schuurink, R., Mauch, F., Buchala, A., Cardinale, F., and Meskiene, I. (2007) The PP2C-Type Phosphatase AP2C1, Which Negatively Regulates MPK4 and MPK6, Modulates Innate Immunity, Jasmonic Acid, and Ethylene Levels in Arabidopsis. The Plant Cell Online 19, 2213-2224.