Dynamic in vivo imaging of cellular parameters in plants combining fluorescent nanosensors and microfluidic platforms
Institute for Integrative Biology of the Cell (I2BC)
Teams : Integrated Approaches to Ion Transport, Plant Cell Signaling and Ubiquitin, Dynamics of Cell Compartmentation in plant cells
Institut Jean-Pierre Bourgin (IJPB)
Teams : Primary Cell Wall, Spatial control of Cell Division, Differenciation and Cell Polarity, Modeling and digital imaging
Institute of Plant Sciences Paris-Saclay (IPS2)
Teams : Regulatory non-coding RNAs in root plasticity, Stress signaling
Laboratoire d’hydrodynamique de l’Ecole Polytechnique (LadHyX)
Abstract of the project
Systems biology approaches in cell biology require a combination of computational modelling with the measurement of cellular processes with a high spatiotemporal resolution in individual cells.
This is facilitated by the recent development of genetically encoded nanosensors reporting cellular parameters.
The DYNANO project addresses two bottlenecks for the adoption of these technologies : the lack of efficient experimental systems for the observation of rapid changes in cellular parameters and the need for data analysis procedures to obtain quantitative information on cellular processes.
The project will be carried out by a multidisciplinary team bringing together more than 15 scientists from the three institutes of the Labex Saclay Plant Science, the hydrodynamics laboratory of the Ecole Polytechnique (LadHyX) and the Elvesys microfluidics company.
DYNANO will develop microfluidic chips for the analysis of response kinetics in parallel in multiple roots and will master developing biosensors technologies. Moreover, DYNANO will develop methodologies for the acquisition and analysis of the data.
DYNANO partners will use the technologies for the analysis of cytosolic and apoplastic Ca2+, ROS and pH, anion fluxes and heavy metal concentrations, cytoskeleton dynamics, ubiquitination, autophagosome formation, MAP kinase activity, mechanosensing, lipid domain formation and plant microbe interactions. DYNANO is expected to reinforce links with the private sector through the development of new generations of microfluidic chips and the possibility to develop platforms for the screening of cellular responses to molecules.
The concerted effort around DYNANO is expected to lead to new scientific breakthroughs in the field of quantitative live cell imaging in plants.