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Genopole Evry

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Accueil > Plateformes > Plateforme Transcriptomique > Available technologies

CATMA v7

CATMAv7 Arabidopsis thaliana

 

 

The selected chip format is 180000 probes per plex on a 4 plex array coming from AGILENT Technology.

 

On the CATMAv7 Agilent array

There will be 38360 probes corresponding to annotated sequences (30834 probes corresponding to TAIRv8 annotation (including 476 probes of mitochondrial and chloroplast genes) + 1289 probes corresponding to EUGENE software predictions. Moreover, we included 5352 probes corresponding to repeat elements, 658 probes for miRNA/MIR, 342 probes for other RNAs (rRNA,tRNA, snRNA, soRNA) and finally 36 controls).
Each annotated probe is triplicated in each plex for robust analysis and in both forward and reverse orientation.
With this new CATMA array, we can perform more hybridizations at the same time, on a more stable support than the previous “in house spotted” array.

 

Material to be supplied by users

  • We require a minimum amount of 2 µg of total RNA of perfect quality per array (RNA extraction using columns such as Qiagen RNeasy for example in DEPC treated water and conserved at -80 ° C.
  • Titration by spectrophotometry with OD 260nm/280nm values between 1.9 and 2.1.
  • The RNAs samples will be sent to us by fast transporters and in dry ice.

 

Price

The total cost : CATMAv7 array + Agilent chip to check the RNA quality (from 1 to 12 samples) + amplification + labelled cDNA+Various consumables (Ribogreen).

- tarifications-pf-2016.pdf

 

Note : We always perform technical (dye-swap/dye-switch) and at least one biological repetition. Why do we perform two colour arrays instead of one colour arrays ?
Please read this document : twocolorvsonecolor.pdf

 

For any project and before sending RNA samples to the platform, please fill the following agreement :
- entente-prealable-catmav7-2016
- agreement-catmav7-2016

 

Contact

Ludivine SOUBIGOU-TACONNAT :

tél : +331.69.15.77.19